CTAGE5 Polyklonaler Antikörper

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• KD/KO Validated

CTAGE5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55279-1-AP

Synonyme

MEA6, MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor

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  WB analysis of HepG2 using 55279-1-AP

  WB result of CTAGE5 antibody (55279-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CTAGE5 transfected HepG2 cells.

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  WB analysis using 55279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 55279-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 55279-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 55279-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HepG2-Zellen, humanes Hirngewebe, humanes Hodengewebe, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55279-1-AP bindet in WB, IHC, ELISA CTAGE5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: CTAGE family, member 5
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: NM_005930
• Gene symbol: CTAGE5
• Gene ID (NCBI): 4253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241). This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B.