CTAGE5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CTAGE5 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55279-1-PBS

Synonyme

MEA6, MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor

---

• 

  WB analysis using 55279-1-AP (same clone as 55279-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 55279-1-AP (same clone as 55279-1-PBS)

  WB result of CTAGE5 antibody (55279-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CTAGE5 transfected HepG2 cells. This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 55279-1-AP (same clone as 55279-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 55279-1-AP (same clone as 55279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 55279-1-AP (same clone as 55279-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55279-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

55279-1-PBS bindet in WB, IHC, Indirect ELISA CTAGE5 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: CTAGE family, member 5
• Berechnetes Molekulargewicht: 92 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: NM_005930
• Gene symbol: CTAGE5
• Gene ID (NCBI): 4253
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241). This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B.