CTBP1 Monoklonaler Antikörper

CTBP1 Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C4B2

Kat-Nr. : 66995-1-PBS

Synonyme

1C4B2, BARS, CTBP, C-terminal-binding protein 1, EC:1.1.1.-

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  WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  WB analysis using 66995-1-Ig (same clone as 66995-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66995-1-Ig (same clone as 66995-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66995-1-Ig (same clone as 66995-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 66995-1-Ig (same clone as 66995-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66995-1-Ig (same clone as 66995-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66995-1-Ig (same clone as 66995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66995-1-Ig (same clone as 66995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66995-1-Ig (same clone as 66995-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66995-1-Ig (same clone as 66995-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66995-1-Ig (same clone as 66995-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66995-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66995-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA CTBP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CTBP1 fusion protein Ag28567
• Vollständiger Name: C-terminal binding protein 1
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 45-48 kDa
• GenBank-Zugangsnummer: BC011655
• Gene symbol: CTBP1
• Gene ID (NCBI): 1487
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.