CTCF Polyklonaler Antikörper

CTCF Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, RIP, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10915-1-AP

Synonyme

11 zinc finger protein, CCCTC binding factor, CTCF, CTCFL paralog, Transcriptional repressor CTCF

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Galerie der Validierungsdaten

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  WB analysis of human brain using 10915-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10915-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of 4T1 using 10915-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 10915-1-AP (CTCF Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of MCF-7 using 10915-1-AP

  IP Result of anti-CTCF (IP:10915-1-AP, 5ug; Detection:10915-1-AP 1:300) with MCF-7 cells lysate 2560ug.

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  IHC staining of human lymphoma using 10915-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10915-1-AP (CTCF antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HepG2 using 10915-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10915-1-AP (CTCF antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, 4T1-Zellen, HEK-293T-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

10915-1-AP bindet in WB, RIP, IP, IHC, IF, ChIP, ELISA CTCF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTCF fusion protein Ag1340
• Vollständiger Name: CCCTC-binding factor (zinc finger protein)
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 55-100 kDa, 130-150 kDa
• GenBank-Zugangsnummer: BC014267
• Gene symbol: CTCF
• Gene ID (NCBI): 10664
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transcriptional insulators are DNA elements that set boundaries on the actions of enhancer and silencer elements and thereby organize the eukaryotic genome into regulatory domains. All vertebrate insulators appear to use the versatile CTCF protein. CTCF uses various combinations of its 11 zinc fingers to recognize a variety of unrelated DNA sequences. Once bound to DNA, CTCF can function as a transcriptional insulator, repressor, or activator, depending on the context of the binding site [PMID:12787766,15454938]. In vertebrates, this 11 zinc-finger protein is shown to be crucial in processes of epigenetic imprinting, X chromosome inactivation , and associated with various complex human diseases including cancer and diabetes [PMID:23139640]. The calcualted molecular weight of CTCF is 83 kDa, but stimulation of human corneal epithelial cells with hypoxic stress suppressed a high molecular mass form of CTCF (150 kDa), but not a lower molecular weight form of CTCF (130 kDa)(PMID: 22354964), and there are multiple isoforms of CTCF with molecular masses of 55, 70, 73, 80, 97, and 130 kDa have been observed (PMID: 12878173).

Publikationen

Species	Application	Title
	RIP	Sci Total Environ; Transcriptome sequencing analysis reveals a potential role of lncRNA NONMMUT058932.2 and NONMMUT029203.2 in abnormal myelin development of male offspring following prenatal PM2.5 exposure; Authors - Yanwen Hou
human	WB	Carcinogenesis; Hepatocellular carcinoma risk variant modulates lncRNA HLA-DQB1-AS1 expression via a long-range enhancer-promoter interaction.; Authors - Haoxue Wang
human	ChIP	mBio; A Low-Producing Haplotype of Interleukin-6 Disrupting CTCF Binding Is Protective against Severe COVID-19.; Authors - Tao Chen