CTDSP2 Polyklonaler Antikörper

CTDSP2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11116-1-AP

Synonyme

CTDSP2, NIF2, NLI interacting factor 2, OS4, Protein OS 4, PSR2, SCP2, Small CTD phosphatase 2

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  WB analysis of human kidney using 11116-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11116-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11116-1-AP (CTDSP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11116-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11116-1-AP (CTDSP2 antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Nierengewebe, HepG2-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11116-1-AP bindet in WB, IHC, ELISA CTDSP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTDSP2 fusion protein Ag1601
• Vollständiger Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 2
• Berechnetes Molekulargewicht: 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC065920
• Gene symbol: CTDSP2
• Gene ID (NCBI): 10106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTDSPs are C-class phosphatases that act on Ser 5 of the Pol II C-terminal domain (CTD) to decrease transcription [PMID:15681389]. CTDSP enzymatic activity is required for REST function, and knockdown of CTDSP induces the neuronal differentiation of mouse embryonic carcinoma P19 cells. CTDSP2 has been shown to enhance TGF-β signaling by the dephosphorylation of SMAD proteins and attenuates androgen receptor-mediated transcription [PMID:16724108]. It negatively regulates RNA polymerase II transcription by controlling the transition from initiation/capping to processive transcript elongation. It was recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells[PMID: 12721286].