CTDSPL2 Polyklonaler Antikörper

CTDSPL2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17532-1-PBS

Synonyme

CTD small phosphatase-like protein 2, CTDSP like 2, CTDSP-like 2, EC:3.1.3.-, HSPC058

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  WB analysis of MCF-7 using 17532-1-AP (same clone as 17532-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse eye using 17532-1-AP (same clone as 17532-1-PBS)

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 17532-1-AP (same clone as 17532-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 17532-1-AP (same clone as 17532-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17532-1-AP (same clone as 17532-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17532-1-AP (CTDSPL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

  IP result of anti-CTDSPL2 (IP:17532-1-AP, 4ug; Detection:17532-1-AP 1:500) with mouse testis tissue lysate 4400ug. This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 17532-1-AP (same clone as 17532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17532-1-AP (CTDSPL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 17532-1-AP (same clone as 17532-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17532-1-AP (CTDSPL2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 17532-1-AP (same clone as 17532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CTDSPL2 antibody (17532-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17532-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17532-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CTDSPL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTDSPL2 fusion protein Ag11581
• Vollständiger Name: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase like 2
• Berechnetes Molekulargewicht: 466 aa, 53 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC035744
• Gene symbol: CTDSPL2
• Gene ID (NCBI): 51496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTD small phosphatase-like protein 2 (CTDSPL2) is also named as HSPC058 and HSPC129, belongs to the CTDSPL2 family. It is a putative RNA-polymerase II carboxy-terminal domain (CTD) phosphatase, whose specific CTD phosphatase activity was verified in vitro, and may regulate the dynamic phosphorylation of RNAP II CTD.( PMID: 17487459) It can obviously improve the expression of ε- and γ-globin genes in K562 cells and CD34+ cells derived from UCB.( PMID: 20932329) This antibody is a rabbit polyclonal antibody raised against a region of human CTDSPL2.