CTLA-4/CD152 Rekombinanter Antikörper

CTLA-4/CD152 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

241100B8

Kat-Nr. : APC-FcA98147

Synonyme

Ctla4, Cd152, Ctla 4, Ly 56

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  FC experiment of mouse splenocytes using APC-FcA98147

  1x10^6 untreated or Con A-treated mouse splenocytes were surface stained with PE Anti-Mouse CD4 (RM4-4) and 0.1 ug APC Anti-Mouse CTLA-4/CD152 Rabbit RecAb (APC-FcA98147, Clone: 241100B8). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using APC-FcA98147

  1x10^6 Con A-treated mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CTLA-4/CD152 Rabbit RecAb (APC-FcA98147, Clone: 241100B8) (red) or 0.1 ug APC Rabbit IgG Isotype Control RecAb (APC-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CTLA-4/CD152 Rabbit RecAb (APC-FcA98147, Clone:241100B8) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Con A treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-FcA98147 bindet in FC CTLA-4/CD152 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CTLA-4/CD152 fusion protein Eg0632
• Vollständiger Name: cytotoxic T-lymphocyte-associated protein 4
• Berechnetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NM_009843.4
• Gene symbol: CTLA-4
• Gene ID (NCBI): 12477
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).