CTLA-4/CD152 Rekombinanter Antikörper

CTLA-4/CD152 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

PE Fluorescent Dye

CloneNo.

240793E8

Kat-Nr. : PE-98078

Synonyme

CTLA4, 240793E8, CD152, CELIAC3, CTLA 4

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  FC experiment of human PBMCs using PE-98078

  1x10^6 unstimulated or PHA-stimulated human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul PE Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (PE-98078, Clone: 240793E8). Cells were not fixed.

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  FC experiment of human PBMCs using PE-98078

  1x10^6 PHA-stimulated human PBMCs were surface stained with 5 ul PE Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (PE-98078, Clone: 240793E8) (red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). 1x10^6 unstimulated human PBMCs were surface stained with 5 ul PE Anti-Human CTLA-4/CD152 Rabbit Recombinant Antibody (PE-98078, Clone: 240793E8) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

PE-98078 bindet in FC CTLA-4/CD152 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CTLA-4/CD152 fusion protein Eg0972
• Vollständiger Name: cytotoxic T-lymphocyte-associated protein 4
• Berechnetes Molekulargewicht: 223 aa, 25 kDa
• GenBank-Zugangsnummer: BC070162
• Gene symbol: CTLA4
• Gene ID (NCBI): 1493
• Konjugation: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses.