p120 Catenin Polyklonaler Antikörper

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• KD/KO Validated

p120 Catenin Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 12180-1-AP

Synonyme

CAS, Catenin delta 1, CTNND, CTNND1, KIAA0384, p120, p120 Catenin, p120(cas), p120(ctn), P120CAS, p120-catenin, P120CTN

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Galerie der Validierungsdaten

• 

  WB analysis of human brain using 12180-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12180-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12180-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 12180-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12180-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12180-1-AP (p120 Catenin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12180-1-AP

  WB result of p120 Catenin antibody (12180-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HeLa cells.

• 

  WB analysis of HEK-293 using 12180-1-AP

  WB result of p120 Catenin antibody (12180-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HEK-293 cells.

• 

  IP experiment of mouse brain using 12180-1-AP

  IP Result of anti-p120 Catenin (IP:12180-1-AP, 4ug; Detection:12180-1-AP 1:500) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human breast cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12180-1-AP (p120 Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12180-1-AP (p120 Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12180-1-AP (p120 Catenin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12180-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12180-1-AP (p120 Catenin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12180-1-AP

  Immunofluorescent analysis of HepG2 cells, using CTNND1 antibody 12180-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, NIH/3T3-Zellen, RAW264.7-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IF: See 2 publications below

Produktinformation

12180-1-AP bindet in WB, IP, IHC, IF, ELISA p120 Catenin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: p120 Catenin fusion protein Ag2824
• Vollständiger Name: catenin (cadherin-associated protein), delta 1
• Berechnetes Molekulargewicht: 948 aa, 105 kDa
• Beobachtetes Molekulargewicht: 90-120 kDa
• GenBank-Zugangsnummer: BC010501
• Gene symbol: CTNND1
• Gene ID (NCBI): 1500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; Inhibition of Sema4D/PlexinB1 signaling alleviates vascular dysfunction in diabetic retinopathy.; Authors - Jie-Hong Wu
mouse	WB	EBioMedicine; NDRG2 regulates adherens junction integrity to restrict colitis and tumourigenesis.; Authors - Mengying Wei
human	WB	Cell Cycle; Hsa-miR-425-5p promotes tumor growth and metastasis by activating the CTNND1-mediated β-catenin pathway and EMT in colorectal cancer.; Authors - Dingsheng Liu
human,mouse	WB	Front Pharmacol; ALG-bFGF Hydrogel Inhibiting Autophagy Contributes to Protection of Blood-Spinal Cord Barrier Integrity via PI3K/Akt/FOXO1/KLF4 Pathway After SCI.; Authors - Renkan Zhang
mouse	WB	Front Mol Neurosci; F-Actin Dysplasia Involved in Organ of Corti Deformity in Gjb2 Knockdown Mouse Model.; Authors - Xiao-Zhou Liu
human	WB	Stem Cells Dev; Laminin-511 activates the human induced pluripotent stem cell survival via α6β1 integrin-Fyn-RhoA-ROCK signaling.; Authors - Yoshiki Nakashima

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