p120 Catenin Monoklonaler Antikörper

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• KD/KO Validated

p120 Catenin Monoklonal Antikörper für WB, IHC, IF/ICC, IF-Fro, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-Fro, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

CloneNo.

2F7H8

Kat-Nr. : 66208-1-Ig

Synonyme

CTNND1, Cadherin-associated Src substrate, CAS, Catenin delta 1, Catenin delta-1

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  WB analysis of fetal human brain using 66208-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66208-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66208-1-Ig

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HeLa cells.

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  WB analysis of HEK-293 using 66208-1-Ig

  WB result of p120 Catenin antibody (66208-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-p120 Catenin transfected HEK-293 cells.

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  WB analysis of A549 using 66208-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66208-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human breast cancer using 66208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse breast cancer using 66208-1-Ig

  Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of mouse breast cancer using 66208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of A431 using 66208-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  IF Staining of HeLa using 66208-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66208-1-Ig(p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, A431-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, Maus-Kolongewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-Fro: mouse breast cancer
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

66208-1-Ig bindet in WB, IHC, IF/ICC, IF-Fro, IP, ELISA p120 Catenin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: p120 Catenin fusion protein Ag2824
• Vollständiger Name: catenin (cadherin-associated protein), delta 1
• Berechnetes Molekulargewicht: 948 aa, 105 kDa
• Beobachtetes Molekulargewicht: 90-120 kDa
• GenBank-Zugangsnummer: BC010501
• Gene symbol: p120 Catenin
• Gene ID (NCBI): 1500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

Publikationen

Species	Application	Title
human	WB,IF,IP	Mol Oncol; Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1.; Authors - Wei Tian
mouse	IF	Development; Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.; Authors - Dianlei Guo
human	IP	Proc Natl Acad Sci U S A; SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.; Authors - Qing Lyu
rat	IF,WB	Biomed Pharmacother; Inhibition of autophagy and RIP1/RIP3/MLKL-mediated necroptosis by edaravone attenuates blood spinal cord barrier disruption following spinal cord injury; Authors - Bo Xu
human	WB	Clin Exp Pharmacol Physiol; Colorectal cancer cells with stably expressed SIRT3 demonstrate proliferating retardation by Wnt/β-catenin cascade inactivation; Authors - Tianyu Li
		Cancer Discov; Spatial Transcriptomics of Intraductal Papillary Mucinous Neoplasms of the Pancreas Identifies NKX6-2 as a Driver of Gastric Differentiation and Indolent Biological Potential; Authors - Marta Sans