CTRL Polyklonaler Antikörper

CTRL Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF,ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 14856-1-AP

Synonyme

chymotrypsin like, Chymotrypsin like protease CTRL 1, CTRL, CTRL1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse pancreas using 14856-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 14856-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14856-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14856-1-AP (CTRL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 14856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 14856-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 14856-1-AP (CTRL antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of BxPC-3 using 14856-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 14856-1-AP (CTRL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: BxPC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14856-1-AP bindet in WB, IHC, IF,ELISA CTRL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTRL fusion protein Ag6644
• Vollständiger Name: chymotrypsin-like
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC063475
• Gene symbol: CTRL
• Gene ID (NCBI): 1506
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.

Publikationen

Species	Application	Title
human	WB	Thromb Haemost; Plasma Proteolytic Cascade Activation during Neonatal Cardiopulmonary Bypass Surgery.; Authors - Susan A Maroney
mouse	WB	Biol Open; Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems.; Authors - Timothy L Hostelley
		Oncotarget; The levels of serine proteases in colon tissue interstitial fluid and serum serve as an indicator of colorectal cancer progression.; Authors - Yingying Xie