Cathepsin A Polyklonaler Antikörper

Cathepsin A Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15020-1-AP

Synonyme

Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, CTSA, GLB2, GSL, Lysosomal protective protein, NGBE, PPCA, PPGB, Protective protein cathepsin A

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Galerie der Validierungsdaten

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  WB analysis using 15020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Sp2/0 using 15020-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 15020-1-AP (Cathepsin A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15020-1-AP (Cathepsin A antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, DU 145-Zellen, PC-3-Zellen, Sp2/0-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Mammakarzinomgewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• FC: See 1 publications below

Produktinformation

15020-1-AP bindet in WB, IHC, FC, ELISA Cathepsin A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin A fusion protein Ag7073
• Vollständiger Name: cathepsin A
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-60, 32, 20 kDa
• GenBank-Zugangsnummer: BC000597
• Gene symbol: CTSA
• Gene ID (NCBI): 5476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).

Publikationen

Species	Application	Title
mouse	WB	J Hepatol; SNX10 mediates alcohol-induced liver injury and steatosis via regulating chaperone-mediated autophagy activation.; Authors - Yan You
human	IHC	Am J Cancer Res; High cathepsin A protein expression predicts poor prognosis and tumor recurrence of hepatocellular carcinoma patients after curative hepatectomy; Authors - Laibang Luo
mouse	WB	Mol Carcinog; Resistance to abemaciclib is associated with increased metastatic potential and lysosomal protein deregulation in breast cancer cells; Authors - Erin R Scheidemann
human	IHC	Sci Rep; Prognostic significance and oncogene function of cathepsin A in hepatocellular carcinoma.; Authors - Huaxiang Wang
mouse	WB	Front Cell Neurosci; Neu1 Is Released From Activated Microglia, Stimulating Microglial Phagocytosis and Sensitizing Neurons to Glutamate.; Authors - David H Allendorf
mouse	FC	Nature; Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.; Authors - Dali Han