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CTSA Rekombinanter Antikörper

CTSA Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251086D9

Kat-Nr. : 80272-3-PBS

Synonyme

cathepsin A, EC:3.4.16.5, GLB2, GSL, Lysosomal protective protein

Filter:
  • Western Blot (WB) analysis of various lysates using CTSA Recombinant antibody (80272-3-RR)

    WB analysis using 80272-3-RR (same clone as 80272-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80272-3-RR (CTSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80272-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80272-3-RR

    Affinity and Kinetic Characterization of 80272-3-RR

    Biolayer interferometry (BLl) kinetic assays of 80272-3-RR against Human CTSA were performed. The affinity constant is 0.717 nM.

Geprüfte Anwendungen

Produktinformation

80272-3-PBS bindet in WB, Indirect ELISA CTSA und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CTSA fusion protein Ag7073
Vollständiger Name cathepsin A
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht 54-60, 32, 20 kDa
GenBank-ZugangsnummerBC000597
Gene symbol Cathepsin A
Gene ID (NCBI) 5476
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).