CTSA Rekombinanter Antikörper

CTSA Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251086D9

Kat-Nr. : 80272-3-RR

Synonyme

cathepsin A, EC:3.4.16.5, GLB2, GSL, Lysosomal protective protein

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  WB analysis using 80272-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80272-3-RR (CTSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 80272-3-RR

  Biolayer interferometry (BLl) kinetic assays of 80272-3-RR against Human CTSA were performed. The affinity constant is 0.717 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, DU 145-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

80272-3-RR bindet in WB, ELISA CTSA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CTSA fusion protein Ag7073
• Vollständiger Name: cathepsin A
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-60, 32, 20 kDa
• GenBank-Zugangsnummer: BC000597
• Gene symbol: Cathepsin A
• Gene ID (NCBI): 5476
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSA is also named as PPGB(protective protein for beta-galactosidase), PPCA(protective protein cathepsin A), cathepsin A, carboxypeptidase C, carboxypeptidase L and belongs to the peptidase S10 family. It is a ubiquitously expressed multifunctional enzyme, with deamidase, esterase, and carboxypeptidase activities and a preference for substrates with hydrophobic amino acid residues at the P1-prime position. It can be cleaved into the following 2 chains and defects in CTSA are the cause of galactosialidosis (GSL). This protein has 2 glycosylation sites. CTSA is synthesized as a 54-kDa precursor protein and composed of 32- and 20-kDa subunits linked together by disulfide bonds(PMID: 16461364,19574551).