Cathepsin B Polyklonaler Antikörper

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• KD/KO Validated

Cathepsin B Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (5)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(87)

Rezensionen (1)

Kat-Nr. : 12216-1-AP

Synonyme

CTSB, APP secretase, APPS, Cat B, Cathepsin B heavy chain

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  WB analysis using 12216-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12216-1-AP (Cathepsin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12216-1-AP

  WB result of Cathepsin B antibody (12216-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cathepsin B transfected HepG2 cells.

• 

  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12216-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12216-1-AP (Cathepsin B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A375 using 12216-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A375 cells using Cathepsin B antibody (12216-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 12216-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human Cathepsin B (12216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HepG2-Zellen, HT-1080.Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen
• Erfolgreiche Detektion in FC (Intra): MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 68 publications below
• IHC: See 8 publications below
• IF: See 21 publications below

Produktinformation

12216-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Cathepsin B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind, Frosch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin B fusion protein Ag2898
• Vollständiger Name: cathepsin B
• Berechnetes Molekulargewicht: 339 aa, 38 kDa
• Beobachtetes Molekulargewicht: 25 kDa, 31 kDa, 43 kDa
• GenBank-Zugangsnummer: BC010240
• Gene symbol: Cathepsin B
• Gene ID (NCBI): 1508
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSB(Cathepsin B) is also named as CPSB and belongs to the peptidase C1 family. It participates in intracellular degradation and turnover of proteins. Cathepsin B precursors found in human malignant ascites fluid do not possess mannose-rich carbohydrates suggesting that a defect in the post translational processing of carbohydrate moieties on tumor. Cathepsin B exists as both glycosylated and unglycosylated forms(PMID:1637335). In rat macrophages and hepatocytes pro- cathepsin B is 39 kDa (unglycosylated =35 kDa), whereas in human fibroblasts procathepsin B is 44.5-46kDa (unglycosylated=39kDa)(PMID:2097084). It can be detected the 43 kDa form of pro-CTSB, 31 kDa and 25 kDa mature forms in mouse brain by western blot(PMID:20616152).

Publikationen

Species	Application	Title
mouse	WB,IF	Nature; Neurotoxic microglia promote TDP-43 proteinopathy in progranulin deficiency.; Authors - Jiasheng Zhang
rat	WB	Cell Death Differ; NLRX1 mediated impaired microglial phagocytosis of NETs in cerebral ischemia and reperfusion injury; Authors - Jialing Peng
human	WB	Autophagy; Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α; Authors - Qinyu Li
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
mouse	WB,IHC	Aging Cell; Growth differentiation factor 11 accelerates liver senescence through the inhibition of autophagy; Authors - Jian Sun
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Weihao (Verified Customer) (02-03-2022)

Works well for mouse BMDM and human macrophages, recommend it

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500