Cathepsin D Polyklonaler Antikörper

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• KD/KO Validated

Cathepsin D Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(143)

Rezensionen (4)

Kat-Nr. : 21327-1-AP

Synonyme

CTSD, Cathepsin D heavy chain, Cathepsin D light chain, CLN10, CPSD

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  WB analysis of HCT 116 using 21327-1-AP

  WB result of Cathepsin D antibody (21327-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and sg-Cathepsin D transfected HCT-116 cells.

• 

  WB analysis using 21327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 21327-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21327-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 21327-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of A431 using 21327-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cathepsin D antibody (21327-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HCT 116-Zellen, HepG2-Zellen, HuH-7-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 123 publications below
• IHC: See 16 publications below
• IF: See 22 publications below

Produktinformation

21327-1-AP bindet in WB, IHC, IF/ICC, ELISA Cathepsin D und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin D fusion protein Ag15254
• Vollständiger Name: cathepsin D
• Berechnetes Molekulargewicht: 412 aa, 45 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 48 kDa, 52 kDa
• GenBank-Zugangsnummer: BC016320
• Gene symbol: Cathepsin D
• Gene ID (NCBI): 1509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease (PMID: 27114232, PMID: 30717773, PMID: 30051532).

Publikationen

Species	Application	Title
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	Acta Pharm Sin B; Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.; Authors - Xiliang Du
human	WB	Autophagy; Atractylenolide I inhibits angiogenesis and reverses sunitinib resistance in clear cell renal cell carcinoma through ATP6V0D2-mediated autophagic degradation of EPAS1/HIF2α; Authors - Qinyu Li
mouse	WB,IF	Redox Biol; Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury; Authors - Yu Xu
mouse,human	WB,IF	Autophagy; Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.; Authors - M Iqbal Hossain ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tae Yeon (Verified Customer) (09-02-2025)

Good puncta staining to detect lysosomal enzyme

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Human, mouse, iPSC-derived organoids

FH

aude (Verified Customer) (05-26-2025)

Sat 5% milk Incubation Primary Ab ON at 4°C

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: 48h conditioned media of TNBC cell lines

Cathepsin D Antibody Western Blot validation (1/10000 dilution) in 48h conditioned media of TNBC cell lines (Cat no:21327-1-AP)

FH

Chen (Verified Customer) (06-01-2021)

Very good to detect cathepsin D in postmortem human samples with Western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: postmortem human brain lysate

Cathepsin D Antibody Western Blot validation (1:3000 dilution) in postmortem human brain lysate (Cat no:21327-1-AP)

FH

Efil (Verified Customer) (05-29-2019)

cells were fixed in cold MeOH for 8 minutes

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human fibroblasts

Cathepsin D Antibody Immunofluorescence, validation (1:100 dilution) in human fibroblasts (Cat no:21327-1-AP)