Cathepsin D Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Cathepsin D Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

97

Rezensionen (2)

Kat-Nr. : 21327-1-AP

Synonyme

cathepsin D, CLN10, CPSD, CTSD

---

Galerie der Validierungsdaten

• 

  WB analysis using 21327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21327-1-AP (Cathepsin D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 21327-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21327-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21327-1-AP (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HepG2-Zellen, HuH-7-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 86 publications below
• IHC: See 13 publications below
• IF: See 12 publications below

Produktinformation

21327-1-AP bindet in WB, IHC, IF, ELISA Cathepsin D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin D fusion protein Ag15254
• Vollständiger Name: cathepsin D
• Berechnetes Molekulargewicht: 412 aa, 45 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 48 kDa, 52 kDa
• GenBank-Zugangsnummer: BC016320
• Gene symbol: CTSD
• Gene ID (NCBI): 1509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CTSD (Cathepsin D) also named CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)

Publikationen

Species	Application	Title
mouse	IF	Acta Neuropathol; Wolframin is a novel regulator of tau pathology and neurodegeneration.; Authors - Shuo Chen
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	Acta Pharm Sin B; Nuciferine protects against high-fat diet-induced hepatic steatosis and insulin resistance via activating TFEB-mediated autophagy-lysosomal pathway.; Authors - Xiliang Du
mouse	WB,IF	Redox Biol; Cyclic helix B peptide alleviates proinflammatory cell death and improves functional recovery after traumatic spinal cord injury; Authors - Yu Xu
human,mouse	WB,IF	Autophagy; Restoration of CTSD (cathepsin D) and lysosomal function in stroke is neuroprotective.; Authors - M Iqbal Hossain ; KD Validated
mouse	WB	Cell Death Differ; Programmed cell death 4 modulates lysosomal function by inhibiting TFEB translation.; Authors - Xiaotong Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Chen (Verified Customer) (06-01-2021)

Very good to detect cathepsin D in postmortem human samples with Western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: postmortem human brain lysate

Cathepsin D Antibody Western Blot validation (1:3000 dilution) in postmortem human brain lysate (Cat no:21327-1-AP)

FH

Efil (Verified Customer) (05-29-2019)

cells were fixed in cold MeOH for 8 minutes

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: human fibroblasts

Cathepsin D Antibody Immunofluorescence, validation (1:100 dilution) in human fibroblasts (Cat no:21327-1-AP)

Verwandte Produkte

CD74 Monoclonal antibody

66390-1-Ig

Cathepsin A Polyclonal antibody

15020-1-AP

BID Polyclonal antibody

10988-1-AP

STK11/LKB1 Polyclonal antibody

10746-1-AP

P53 Polyclonal antibody

21891-1-AP