Cathepsin S Polyklonaler Antikörper

Cathepsin S Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27538-1-AP

Synonyme

CTSS, EC:3.4.22.27

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  WB analysis using 27538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27538-1-AP (Cathepsin S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27538-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27538-1-AP (Cathepsin S antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 27538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (Cathepsin S antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 27538-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (Cathepsin S antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-87 MG using 27538-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using Cathepsin S antibody (27538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, THP-1-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

27538-1-AP bindet in WB, IHC, IF/ICC, ELISA Cathepsin S und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cathepsin S fusion protein Ag25881
• Vollständiger Name: cathepsin S
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 25 kDa, 37 kDa
• GenBank-Zugangsnummer: BC002642
• Gene symbol: CTSS
• Gene ID (NCBI): 1520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cathepsin S (CTSS) is a cysteine protease that plays an important role in extracellular matrix degradation and remodeling, antigen presentation, inflammation, and angiogenesis; it has been identified as a radiation response gene. In malignant tumors, CTSS induces tumor proliferation, invasion and metastasis through various mechanisms. CTSS proteins could be detected in two forms, pro-CTSS (35-37 kDa) and active CTSS (25 kDa) (PMID: 30006610, PMID: 34208434). And in this publication, it was also reporeted that active CTSS was expressed at higher levels than pro-CTSS in the lysate (PMID: 34208434).