Cortactin Polyklonaler Antikörper

Cortactin Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 11381-1-AP

Synonyme

Amplaxin, cortactin, CTTN, EMS1, Oncogene EMS1, Src substrate cortactin

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Galerie der Validierungsdaten

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  WB analysis using 11381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11381-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11381-1-AP

  IP result of anti-Cortactin (IP:11381-1-AP, 4ug; Detection:11381-1-AP 1:1000) with MCF-7 cells lysate 2400 ug.

• 

  IHC staining of human breast cancer using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 11381-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11381-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cortactin antibody (11381-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 11381-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Cortactin antibody (11381-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, HeLa-Zellen, HT-1080.Zellen, HUVEC-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

11381-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Cortactin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cortactin fusion protein Ag1940
• Vollständiger Name: cortactin
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 70-85 kDa
• GenBank-Zugangsnummer: BC008799
• Gene symbol: CTTN
• Gene ID (NCBI): 2017
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

Publikationen

Species	Application	Title
mouse	IF	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	IF	Adv Healthc Mater; Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration; Authors - Zhao Wei
human	WB	EMBO J; TFAM loss induces nuclear actin assembly upon mDia2 malonylation to promote liver cancer metastasis.; Authors - Qichao Huang
human	IHC	Front Oncol; A four oxidative stress gene prognostic model and integrated immunity-analysis in pancreatic adenocarcinoma; Authors - Hao Wang
rat	WB	Neuroreport; Decreased expression of cortactin in the schizophrenia brain.; Authors - Hriday P Bhambhvani

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