CTTNBP2 Polyklonaler Antikörper

CTTNBP2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17893-1-AP

Synonyme

C7orf8, Cortactin-binding protein 2, CortBP2, KIAA1758, Orf4

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  WB analysis of mouse brain using 17893-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17893-1-AP (CTTNBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17893-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17893-1-AP (CTTNBP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17893-1-AP

  IP result of anti-CTTNBP2 (IP:17893-1-AP, 5ug; Detection:17893-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human testis using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17893-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17893-1-AP (CTTNBP2 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausnierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Hirngewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Plazenta-Gewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

17893-1-AP bindet in WB, IHC, IF, IP, ELISA CTTNBP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CTTNBP2 fusion protein Ag12219
• Vollständiger Name: cortactin binding protein 2
• Berechnetes Molekulargewicht: 1663 aa, 181 kDa
• Beobachtetes Molekulargewicht: 181 kDa
• GenBank-Zugangsnummer: BC106000
• Gene symbol: CTTNBP2
• Gene ID (NCBI): 83992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cortactin Binding Protein 2 (CTTNBP2) is a cytoskeleton regulator predominantly expressed in neurons and highly enriched at dendritic spines. CTTNBP2 controls the synaptic expression of a set of zinc-regulated autism-associated genes and influences NMDAR function and signaling (PMID: 32492416).

Publikationen

Species	Application	Title
mouse	WB	Nat Neurosci; Social deficits in Shank3-deficient mouse models of autism are rescued by histone deacetylase (HDAC) inhibition.; Authors - Luye Qin
human	IF	Dev Cell; Single-cell MultiOmics and spatial transcriptomics demonstrate neuroblastoma developmental plasticity; Authors - Yunyun Xu
human	IHC	Mod Pathol; Expression profiling of small intestinal neuroendocrine tumors identifies subgroups with clinical relevance, prognostic markers and therapeutic targets.; Authors - Ellinor Andersson
human	IF	Sci Adv; Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression; Authors - Hao Zhang