CUEDC2 Rekombinanter Antikörper

CUEDC2 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251583E6

Kat-Nr. : 86610-2-PBS

Synonyme

C10orf66, CUE domain containing 2, CUE domain-containing protein 2, HOYS6

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  WB analysis using 86610-2-RR (same clone as 86610-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86610-2-RR (CUEDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86610-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 86610-2-RR (same clone as 86610-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86610-2-RR (CUEDC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86610-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02553-1

  The mean CUEDC2 concentration was determined to be 51.3 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load, 49.9 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP02553-1

  Cytometric bead array standard curve of MP02553-1, CUEDC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86610-1-PBS. Detection antibody: 86610-2-PBS. Standard: Ag13847. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 86610-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86610-2-RR against Human CUEDC2 were performed. The affinity constant is 0.925 nM.

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Geprüfte Anwendungen

Produktinformation

86610-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA CUEDC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CUEDC2 fusion protein Ag13847
• Vollständiger Name: CUE domain containing 2
• Berechnetes Molekulargewicht: 287 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000262
• Gene symbol: CUEDC2
• Gene ID (NCBI): 79004
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CUE domain-containing 2 (CUEDC2) is a ubiquitin-binding motif-containing protein involved in the regulation of the cell cycle, inflammation, and tumorigenesis. It is ubiquitously expressed in human tissues and organs, not only highly expressed in the normal brain, heart and testis, but also some kinds of cancers, such as breast, ovarian and kidney cancers. CUEDC2 could act as an adaptor protein to target IκB kinase (IKK) for dephosphorylation and inactivation by recruiting protein phosphatase (PP1), and thus repressed activation of the transcription factor NF-κB. Moreover, CUEDC2, is a key factor in endocrine resistance in breast cancer.