CUL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CUL1 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Rezensionen (1)

Kat-Nr. : 12895-1-AP

Synonyme

CUL-1, Cullin1, Cullin-1

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• 

  WB analysis using 12895-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12895-1-AP (CUL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 12895-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12895-1-AP (CUL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12895-1-AP

  IP result of anti-CUL1 (IP:12895-1-AP, 4ug; Detection:12895-1-AP 1:2000) with K-562 cells lysate 1760 ug.

• 

  IP experiment of HeLa using 12895-1-AP

  IP result of anti-CUL1 (IP:12895-1-AP, 3ug; Detection:12895-1-AP 1:300) with HeLa cells lysate 2000ug.

• 

  IHC staining of human lung cancer using 12895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12895-1-AP (CUL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12895-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12895-1-AP (CUL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 12895-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CUL1 (12895-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, BxPC-3-Zellen, K-562-Zellen
• Erfolgreiche IP: K-562-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12895-1-AP bindet in WB, IHC, FC (Intra), IP, CoIP, ELISA CUL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUL1 fusion protein Ag3585
• Vollständiger Name: cullin 1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC034318
• Gene symbol: CUL1
• Gene ID (NCBI): 8454
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Publikationen

Species	Application	Title
human	WB	Nat Chem Biol; E2-Ub-R74G strategy reveals E2-specific ubiquitin conjugation profiles in live cells; Authors - Siqi Shen
mouse	WB	Redox Biol; Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.; Authors - Ji Zhang
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
mouse	WB,IP	Pharmacol Res; Cullin-associated and neddylation-dissociated 1 protein (CAND1) governs cardiac hypertrophy and heart failure partially through regulating calcineurin degradation.; Authors - Xingda Li
human	WB	iScience; A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.; Authors - Muzammal Hussain
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

strong band for predicted band

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart