CUL3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CUL3 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (1)

Kat-Nr. : 11107-1-AP

Synonyme

CUL 3, CUL3, cullin 3, KIAA0617

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Galerie der Validierungsdaten

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  WB analysis using 11107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11107-1-AP (CUL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11107-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11107-1-AP (CUL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11107-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11107-1-AP (CUL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11107-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11107-1-AP (CUL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 11107-1-AP

  Immunofluorescent analysis of HEK-293 cells using 11107-1-AP (CUL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maushirngewebe, Maushodengewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenhodengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

11107-1-AP bindet in WB, IP, IHC, IF, ELISA CUL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUL3 fusion protein Ag1555
• Vollständiger Name: cullin 3
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 80-89 kDa
• GenBank-Zugangsnummer: BC039598
• Gene symbol: CUL3
• Gene ID (NCBI): 8452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); DDRGK1 Enhances Osteosarcoma Chemoresistance via Inhibiting KEAP1-Mediated NRF2 Ubiquitination; Authors - Xin Wang
human	WB	Sci Total Environ; USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway.; Authors - Lei Zhang
human	WB	Food Chem; Tangeretin maintains antioxidant activity by reducing CUL3 mediated NRF2 ubiquitination.; Authors - Yue Wang
human	WB	Aging (Albany NY); Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.; Authors - Xiao Dong ; KD Validated
human	WB,IF,IP	FEBS J; CUL3 induces mitochondrial dysfunction via MRPL12 ubiquitination in renal tubular epithelial cells; Authors - Xingzhao Ji
human	WB	Sci Rep; H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression.; Authors - Xing-Hui Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Brittney (Verified Customer) (11-26-2018)

10ug load of protein. Cells lysed in RIPA buffer then added to 2X Laemmli buffer for denaturing. Heated at 95deg for 10 minutes as well.Multiple bands, but all follow same pattern. Assumed the band at ~75 kD was the correct one.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HK-2 Cells

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