CUL3 Polyklonaler Antikörper

CUL3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28087-1-PBS

Synonyme

CUL-3, Cullin-3, KIAA0617

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  WB analysis using 28087-1-AP (same clone as 28087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28087-1-AP (CUL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28087-1-AP (same clone as 28087-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28087-1-AP (CUL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 28087-1-AP (same clone as 28087-1-PBS)

  IP result of anti-CUL3 (IP:28087-1-AP, 4ug; Detection:28087-1-AP 1:600) with mouse testis tissue lysate 1680 ug. This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 28087-1-AP (same clone as 28087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28087-1-AP (CUL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 28087-1-AP (same clone as 28087-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28087-1-AP (CUL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28087-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28087-1-PBS bindet in WB, IHC, IP, Indirect ELISA CUL3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUL3 fusion protein Ag28004
• Vollständiger Name: cullin 3
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 80-88 kDa
• GenBank-Zugangsnummer: BC039598
• Gene symbol: CUL3
• Gene ID (NCBI): 8452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.