CUL3 Rekombinanter Antikörper

CUL3 Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

240534C7

Kat-Nr. : 83619-5-RR

Synonyme

240534C7, CUL-3, Cullin-3, KIAA0617

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  WB analysis using 83619-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83619-5-RR (CUL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 83619-5-RR

  IP result of anti-CUL3 (IP:83619-5-RR, 4ug; Detection:83619-5-RR 1:4000) with HeLa cells lysate 1200 ug.

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  Affinity and Kinetic Characterization of 83619-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83619-5-RR against Human CUL3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, PC-12-Zellen, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83619-5-RR bindet in WB, IP, ELISA CUL3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CUL3 fusion protein Ag1555
• Vollständiger Name: cullin 3
• Berechnetes Molekulargewicht: 89 kDa
• Beobachtetes Molekulargewicht: 80-89 kDa
• GenBank-Zugangsnummer: BC039598
• Gene symbol: CUL3
• Gene ID (NCBI): 8452
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ligase are the largest family of ubiquitin ligases which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. One of seven cullin proteins serves as a scaffold protein for the assembly of the multisubunit ubiquitin ligase complex. CUL3 (Cullin-3) ubiquitin ligase complex targets multiple substrate for ubiquitination including cyclin E and of cyclin D1. Defects in CUL3 are the cause of Pseudohypoaldosteronism type 2E (PHA2E) characterized by severe hypertension, hyperkalemia, hyperchloremia, hyperchloremic metabolic acidosis, and correction of physiologic abnormalities by thiazide diuretics.