CUL4B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CUL4B Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20882-1-AP

Synonyme

KIAA0695, Cullin-4B, cullin 4B

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  WB analysis using 20882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 20882-1-AP

  WB result of CUL4B antibody (20882-1-AP, 1:500) with si-Control and si-CUL4B transfected HeLa cells.

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  WB analysis using 20882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 20882-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 20882-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20882-1-AP

  IP result of anti-CUL4B (IP:20882-1-AP, 4ug; Detection:20882-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human tonsillitis using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 20882-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20882-1-AP (CUL4B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 20882-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (20882-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 20882-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CUL4B antibody (20882-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HeLa using 20882-1-AP

  Immunofluorescent analysis of Hela cells, using CUL4B antibody 20882-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20882-1-AP

  Immunofluorescent analysis of HepG2 cells, using CUL4B antibody 20882-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, HeLa-Zellen, Jurkat-Zellen, Ratten-Thymusgewebe, SH-SY5Y-Zellen, Y79-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

20882-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CUL4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUL4B fusion protein Ag15024
• Vollständiger Name: cullin 4B
• Berechnetes Molekulargewicht: 913 aa, 104 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC036216
• Gene symbol: CUL4B
• Gene ID (NCBI): 8450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329). Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.

Publikationen

Species	Application	Title
	IP	Neuropsychopharmacology; Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.; Authors - Gonca Bayraktar ; KD Validated
mouse	IP	Front Immunol; PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.; Authors - Nianyin Lv
mouse	IF	Cells; Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte Migration, Proliferation and Blood-Testis Barrier Homeostasis.; Authors - Yan Yin