CUL4B Monoklonaler Antikörper

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CUL4B Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4E10B10

Kat-Nr. : 60151-1-PBS

Synonyme

4E10B10, cullin 4B, Cullin-4B, KIAA0695

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  WB analysis using 60151-1-Ig (same clone as 60151-1-PBS)

  various lysates were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa cells using 60151-1-Ig (same clone as 60151-1-PBS)

  WB result of CUL4B antibody (60151-1-Ig, 1:500) with si-Control and si-CUL4B transfected HeLa cells. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60151-1-Ig (same clone as 60151-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60151-1-Ig (same clone as 60151-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60151-1-Ig (same clone as 60151-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60151-1-Ig (CUL4B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 60151-1-Ig (same clone as 60151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60151-1-Ig (CUL4B Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60151-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60151-1-PBS bindet in WB, IHC, Indirect ELISA CUL4B und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CUL4B fusion protein Ag3563
• Vollständiger Name: cullin 4B
• Berechnetes Molekulargewicht: 913 aa, 104 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC036216
• Gene symbol: CUL4B
• Gene ID (NCBI): 8450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).