CUL4B Rekombinanter Antikörper

CUL4B Rekombinant Antikörper für IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242626F11

Kat-Nr. : 85001-4-PBS

Synonyme

242626F11, cullin 4B, Cullin-4B, KIAA0695

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  IF Staining of HeLa using 85001-4-RR (same clone as 85001-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUL4B antibody (85001-4-RR, Clone: 242626F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85001-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 85001-4-RR (same clone as 85001-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CUL4B Recombinant antibody (85001-4-RR, Clone:242626F11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85001-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85001-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85001-4-RR against Human CUL4B were performed. The affinity constant is 0.51 nM.

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Geprüfte Anwendungen

Produktinformation

85001-4-PBS bindet in IF/ICC, FC (Intra), Indirect ELISA CUL4B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CUL4B fusion protein Ag3563
• Vollständiger Name: cullin 4B
• Berechnetes Molekulargewicht: 913 aa, 104 kDa
• GenBank-Zugangsnummer: BC036216
• Gene symbol: CUL4B
• Gene ID (NCBI): 8450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cullin-RING ligases (CRLs) complexes participate in the regulation of diverse cellular processes, including cell cycle progression, transcription, signal transduction and development (PMID: 21816341)(PMID: 21554755). Serving as the scaffold protein, cullins are crucial for the assembly of ligase complexes, which recognize and target various substrates for proteosomal degradation. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), are two members in cullin family with 83% of identity. Mutations in human CUL4B are one of the major causes of X-linked mental retardation. Cul4b knockout mice demonstrated that CUL4B is indispensable for embryonic development in the mouse (PMID: 22606329).