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Cullin 5 Monoklonaler Antikörper

Cullin 5 Monoklonal Antikörper für ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

ELISA

Konjugation

Unkonjugiert

CloneNo.

4A3E9

Kat-Nr. : 60093-1-Ig

Synonyme

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of Y79 cells using Cullin 5 Monoclonal antibody (60093-1-Ig)

    WB analysis of Y79 using 60093-1-Ig

    Y79 cells were subjected to SDS PAGE followed by western blot with 60093-1-Ig (Cullin 5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using Cullin 5 Monoclonal antibody (60093-1-Ig)

    IHC staining of human skin using 60093-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin slide using 60093-1-Ig (Cullin 5 antibody at dilution of 1:50 (under 40x lens).

Geprüfte Anwendungen

Empfohlene Verdünnung

AnwendungVerdünnung
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60093-1-Ig bindet in ELISA Cullin 5 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen Cullin 5 fusion protein Ag5453
Vollständiger Name cullin 5
Berechnetes Molekulargewicht 91 kDa
GenBank-ZugangsnummerBC063306
Gene symbol CUL5
Gene ID (NCBI) 8065
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Protokolle

Produktspezifische Protokolle
WB protocol for Cullin 5 antibody 60093-1-IgProtokoll herunterladen
IHC protocol for Cullin 5 antibody 60093-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen