CUX1 Polyklonaler Antikörper

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• KD/KO Validated

CUX1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11733-1-PBS

Synonyme

COY1, Clox, CDP1, CDP/Cut, CDP

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  WB analysis of human brain using 11733-1-AP (same clone as 11733-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  WB analysis using 11733-1-AP (same clone as 11733-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 11733-1-AP (same clone as 11733-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 11733-1-AP (same clone as 11733-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 11733-1-AP (same clone as 11733-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 11733-1-AP (same clone as 11733-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  IP result of anti-CUX1 (IP:11733-1-AP, 4ug; Detection:11733-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 11733-1-AP (same clone as 11733-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CUX1 antibody (11733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse cortex using 11733-1-AP (same clone as 11733-1-PBS)

  IF result of the antibody CUX1 (11733-1-AP, 1:300) with mouse cortex, Data from Dr. Lee O. Vaasjo Muñoz, University of Chicago. . This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11733-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CUX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CUX1 fusion protein Ag2335
• Vollständiger Name: cut-like homeobox 1
• Berechnetes Molekulargewicht: 676 aa, 77 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC025422
• Gene symbol: CUX1
• Gene ID (NCBI): 1523
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).