CUX2 Rekombinanter Antikörper

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• KD/KO Validated

CUX2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

230235G1

Kat-Nr. : 82933-1-RR

Synonyme

Homeobox protein cux-2, Homeobox protein cut-like 2, CUTL2, cut like homeobox 2, CDP2

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  WB analysis using 82933-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82933-1-RR (CUX2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 82933-1-RR

  WB result of CUX2 antibody (82933-1-RR; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUX2 transfected SH-SY5Y cells.

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  IF Staining of HeLa using 82933-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 82933-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CUX2 antibody (82933-1-RR, Clone: 230235G1 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  Affinity and Kinetic Characterization of 82933-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82933-1-RR against Human CUX2 were performed. The affinity constant is 1.02 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HepG2-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82933-1-RR bindet in WB, IF/ICC, ELISA CUX2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CUX2 fusion protein Ag18809
• Vollständiger Name: cut-like homeobox 2
• Berechnetes Molekulargewicht: 1486 aa, 162 kDa
• Beobachtetes Molekulargewicht: 162 kDa
• GenBank-Zugangsnummer: BC151245
• Gene symbol: CUX2
• Gene ID (NCBI): 23316
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cut like homeobox 2 (CUX2) also named as, CDP2 or CUTL2, is a 1486 amino acid protein, which contains one homeobox DNA-binding domain and three CUT DNA-binding domains. CUX2 localizes in the nucleus and belongs to the CUT homeobox family. CUX2 may as a transcription factor be involved in neural specification.