CX3CL1 Polyklonaler Antikörper

CX3CL1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 10108-2-AP

Synonyme

ABCD 3, C X3 C motif chemokine 1, C3Xkine, CX3CL1, CXC3, CXC3C, FKN, fractalkine, neurotactin, NTN, NTT, SCYD1, Small inducible cytokine D1

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Galerie der Validierungsdaten

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  WB analysis of PC-3 using 10108-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10108-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10108-2-AP (CX3CL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens)..

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  IHC staining of human small intestine using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens)..

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  IHC staining of human lung using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 10108-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10108-2-AP (CX3CL1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Dünndarmgewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below

Produktinformation

10108-2-AP bindet in WB, IHC, ELISA CX3CL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CX3CL1 fusion protein Ag0161
• Vollständiger Name: chemokine (C-X3-C motif) ligand 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa
• GenBank-Zugangsnummer: BC001163
• Gene symbol: CX3CL1
• Gene ID (NCBI): 6376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chemokines direct the trafficking of white blood cells in immune surveillance, playing a key role in inflammatory and infectious diseases such as AIDS. The human CX3C chemokine carries the chemokine domain on top of an extended mucin-like stalk. CX3CL1 is synthesized as a 50-75 kDa precursor and undergoes glycosylation to yield two mature forms: a 100 kDa cell membrane bound form and an 85 kDa soluble form which is released from the cell surface. The soluble CX3C chemokine has potent chemoattractant activity for T cells and monocytes, and the cell-surface-bound protein, which is induced by primary proinflammatory signals in activated primary endothelial cells, promotes strong adhesion of those leukocytes.

Publikationen

Species	Application	Title
human	IHC	Blood; Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.; Authors - Ma Yue Y
human	IHC	J Pathol; Current smoking-specific gene expression signature in normal bronchial epithelium is enhanced in squamous cell lung cancer.; Authors - Boelens Mirjam C MC
human	WB	Mol Oncol; Platelets are recruited to hepatocellular carcinoma tissues in a CX3CL1-CX3CR1 dependent manner and induce tumour cell apoptosis.; Authors - Shuo Miao
human	WB	Int J Biol Sci; Long noncoding RNA SH3PXD2A-AS1 promotes colorectal cancer progression by regulating p53-mediated gene transcription.; Authors - Pingfu Hou
human	WB	J Neuropathol Exp Neurol; Immunohistochemical and Molecular Investigations Show Alteration in the Inflammatory Profile of Multiple System Atrophy Brain.; Authors - Aoife P Kiely
human	WB,IHC	Cell Biochem Funct; Fractalkine/CX3CR1 induces apoptosis resistance and proliferation through the activation of the AKT/NF-κB cascade in pancreatic cancer cells.; Authors - Hui Wang

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