CXADR Rekombinanter Antikörper

CXADR Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250811C8

Kat-Nr. : 86186-1-RR

Synonyme

CAR, Coxsackievirus B-adenovirus receptor, CVB3 binding protein, CVB3-binding protein, HCAR

---

• 

  WB analysis using 86186-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86186-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86186-1-RR (CXADR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 86186-1-RR

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 86186-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86186-1-RR (CXADR antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 86186-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86186-1-RR against Human CXADR were performed. The affinity constant is 96.1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HaCaT-Zellen, BxPC-3-Zellen, Mausherzgewebe, Maus-Dünndarmgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86186-1-RR bindet in WB, IHC, ELISA CXADR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXADR fusion protein Ag34346
• Vollständiger Name: coxsackie virus and adenovirus receptor
• Berechnetes Molekulargewicht: 365 aa, 40 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC003684
• Gene symbol: CXADR
• Gene ID (NCBI): 1525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coxsackievirus and adenovirus receptor (CXADR) is a member of junctional adhesion molecules, which are glycosylated transmembrane proteins that belong to the Ig superfamily (PMID: 17916321). CXADR plays essential roles in blastocyst formation, including cavitation and the proper subcellular localization of tight junction proteins into the apical edge of the outer blastomeres (PMID: 26729920). It is also involved in the transepithelial migration of leukocytes through adhesive interactions with junctional adhesion molecule-like protein (JAML) (PMID: 15800062).