CXCL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CXCL1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA, Cell treatment

Konjugation

Unkonjugiert

Publikationen(80)

Kat-Nr. : 12335-1-AP

Synonyme

C X C motif chemokine 1, C-X-C motif chemokine 1, CXCL 1, GRO-alpha(4-73), GRO-alpha(5-73)

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  WB analysis using 12335-1-AP

  LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 12335-1-AP (CXCL1 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12335-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12335-1-AP (CXCL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12335-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12335-1-AP (CXCL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS und Proteintransportinhibitor behandelte HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 27 publications below
• IHC: See 42 publications below
• IF: See 24 publications below
• ELISA: See 3 publications below

Produktinformation

12335-1-AP bindet in WB, IHC, IF, ELISA, Cell treatment CXCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL1 fusion protein Ag2917
• Vollständiger Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Berechnetes Molekulargewicht: 8 kDa to 14 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC011976
• Gene symbol: CXCL1
• Gene ID (NCBI): 2919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Single-cell multi-stage spatial evolutional map of esophageal carcinogenesis; Authors - Jiang Chang
	IHC	Cell Host Microbe; Induction of bone loss by pathobiont-mediated Nod1 signaling in the oral cavity.; Authors - Jiao Yizu Y
human	IHC	J Exp Med; An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition.; Authors - Helen L Young
human	WB,IF	Cell Rep; Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer; Authors - Xinyu Yang ; KD Validated
	IHC	Cell Rep; Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models; Authors - Jiali Dong
	WB,IF	Cell Mol Life Sci; Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion; Authors - Shiju Ye