CXCL1 Polyklonaler Antikörper

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• KD/KO Validated

CXCL1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

45

Kat-Nr. : 12335-1-AP

Synonyme

C X C motif chemokine 1, CXCL1, FSP, GRO, GRO alpha(1 73), GRO1, GROa, Growth regulated alpha protein, MGSA, MGSA a, NAP 3, SCYB1

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Galerie der Validierungsdaten

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  WB analysis using 12335-1-AP

  LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 12335-1-AP (CXCL1 antibody) at dilution of 1:1000 and incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 12335-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12335-1-AP (CXCL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12335-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12335-1-AP (CXCL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS und Proteintransportinhibitor behandelte HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• IHC: See 23 publications below
• IF: See 16 publications below
• ELISA: See 2 publications below

Produktinformation

12335-1-AP bindet in WB, IHC, IF, ELISA CXCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL1 fusion protein Ag2917
• Vollständiger Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Berechnetes Molekulargewicht: 8 kDa to 14 kDa
• GenBank-Zugangsnummer: BC011976
• Gene symbol: CXCL1
• Gene ID (NCBI): 2919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.

Publikationen

Species	Application	Title
mouse	IHC	Cell Host Microbe; Induction of bone loss by pathobiont-mediated Nod1 signaling in the oral cavity.; Authors - Jiao Yizu Y
human	IHC	J Exp Med; An adaptive signaling network in melanoma inflammatory niches confers tolerance to MAPK signaling inhibition.; Authors - Helen L Young
human,mouse	IF	Cell Rep; Collagen 1-mediated CXCL1 secretion in tumor cells activates fibroblasts to promote radioresistance of esophageal cancer; Authors - Xinyu Yang ; KD Validated
mouse	IHC	Cell Rep; Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models; Authors - Jiali Dong
mouse	IF	Cell Mol Life Sci; Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion; Authors - Shiju Ye
human, mouse	IHC	Kidney Int; Discovery of new glomerular disease-relevant genes by translational profiling of podocytes in vivo.; Authors - Ivica Grgic

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