CXCL1 Rekombinanter Antikörper

CXCL1 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240990E5

Kat-Nr. : 83937-1-PBS

Synonyme

C X C motif chemokine 1, C-X-C motif chemokine 1, CXCL 1, GRO-alpha(4-73), GRO-alpha(5-73)

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  Sandwich ELISA standard curve of MP00922-2

  Sandwich ELISA standard curve of MP00922-2, Human CXCL1 Recombinant Matched Antibody Pair - PBS only. 83937-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2917. 83937-2-PBS was biotinylated as the detection antibody. Range: 31.25-2000 pg/mL

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  Sample test of MP00922-2

  Serum of tweleve individual healthy human donors was measured. The human CXCL1 concentration of detected samples was determined to be 110.0 pg/mL with a range of 0.6 - 1,164.0 pg/mL.

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  Sample test of MP00922-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean CXCL1 concentration was determined to be 32.4 pg/mL in unstimulated PBMC supernatant, 9,728.4 pg/mL in PHA stimulated PBMC supernatant.

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  Affinity and Kinetic Characterization of 83937-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83937-1-PBS against Human CXCL1 was performed. The affinity constant is 0.278 nM.

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Geprüfte Anwendungen

Produktinformation

83937-1-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CXCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL1 fusion protein Ag2917
• Vollständiger Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Berechnetes Molekulargewicht: 8 kDa to 14 kDa
• GenBank-Zugangsnummer: BC011976
• Gene symbol: CXCL1
• Gene ID (NCBI): 2919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.