CXCL1 Rekombinanter Antikörper

CXCL1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240990E4

Kat-Nr. : 83937-3-RR

Synonyme

GRO-alpha(5-73), GRO-alpha(4-73), CXCL 1, C-X-C motif chemokine 1, C X C motif chemokine 1

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  WB analysis of HUVEC using 83937-3-RR

  Untreated, LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83937-3-RR (CXCL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83937-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83937-3-RR against Human CXCL1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83937-3-RR bindet in WB, ELISA CXCL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL1 fusion protein Ag2917
• Vollständiger Name: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
• Berechnetes Molekulargewicht: 8 kDa to 14 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: BC011976
• Gene symbol: CXCL1
• Gene ID (NCBI): 2919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL1 a is a member of CXC family and also known as keratinocyte-derived chemokines (KC) or growth-related oncogene (GRO). CXCL1 is expressed by macrophages, neutrophils and epithelial cells. CXCL1 binding specifically to the CXC chemokine receptor CXCR2, is involved in fibrogenesis and angiogenesis. This protein also plays a role in inflammation and as a chemoattractant for neutrophils. CXCL1 is upregulated in some types of human cancer, including colorectal, bladder, breast, prostate and skin cancers.