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CXCL10/IP-10 Rekombinanter Antikörper

CXCL10/IP-10 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250120A12

Kat-Nr. : 85307-2-PBS

Synonyme

CXCL10, CXCL10/IP10, IP10, 10 kDa interferon gamma-induced protein, C X C motif chemokine 10

Filter:
  • Western Blot (WB) analysis of THP-1 cells using CXCL10/IP-10 Recombinant monoclonal antibody (85307-2-RR)

    WB analysis of THP-1 using 85307-2-RR (same clone as 85307-2-PBS)

    IFN gamma, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 85307-2-RR (CXCL10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85307-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CXCL10/IP-10 Recombinant monoclonal antibody (85307-2-RR)

    IF Staining of THP-1 using 85307-2-RR (same clone as 85307-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN gamma, LPS and Brefeldin A treated THP-1 cells using CXCL10/IP-10 antibody (85307-2-RR, Clone: 250120A12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85307-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85307-2-PBS bindet in WB, IF/ICC, Indirect ELISA CXCL10/IP-10 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CXCL10/IP-10 fusion protein Eg2489
Vollständiger Name chemokine (C-X-C motif) ligand 10
Berechnetes Molekulargewicht 11 kDa
Beobachtetes Molekulargewicht11 kDa
GenBank-ZugangsnummerBC010954
Gene symbol CXCL10
Gene ID (NCBI) 3627
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL10 (also known as IP-10) is a member of the CXC chemokine family which binds to the CXCR3 receptor to exert its biological effects. CXCL10 is a 12-kDa protein and constitutes two internal disulfide cross bridges. The predicted signal peptidase cleavage generates a 10-kDa secreted polypeptide with four conserved cysteine residues in the N-terminal. The CXCL10 gene localizes on chromosome 4 at band q21, a locus associated with an acute monocytic/B-lymphocyte lineage leukemia exhibiting translocation of t (4; 11) (q21; q23). CXCL10 mediates leukocyte trafficking, adaptive immunity, inflammation, haematopoiesis and angiogenesis. Under proinflammatory conditions CXCL10 is secreted from a variety of cells, such as leukocytes, activated neutrophils, eosinophils, monocytes, epithelial cells, endothelial cells, stromal cells (fibroblasts) and keratinocytes in response to IFN-γ.