CXCL13/BCA1 Polyklonaler Antikörper

CXCL13/BCA1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(26)

Kat-Nr. : 10927-1-AP

Synonyme

CXCL13, chemokine (C X C motif) ligand 13, C X C motif chemokine 13, BCA-1, BCA1

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  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10927-1-AP (CXCL13/BCA1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 10927-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 10927-1-AP (CXCL13/BCA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of TT using 10927-1-AP

  1x10^6 TT cells were intracellularly stained with 0.2 ug CXCL13/BCA1 Polyclonal antibody (10927-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes Lebergewebe, humanes Lymphomgewebe, humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): TT cells

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 15 publications below
• IF: See 6 publications below

Produktinformation

10927-1-AP bindet in WB, IHC, IF, ELISA CXCL13/BCA1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL13/BCA1 fusion protein Ag1358
• Vollständiger Name: chemokine (C-X-C motif) ligand 13
• Berechnetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC012589
• Gene symbol: CXCL13/BCA1
• Gene ID (NCBI): 10563
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BLR1 (Burkitt lymphoma receptor-1), also called CXCR5, is highly expressed on Burkitt lymphoma cells and B lymphocytes. It may therefore function in the homing of B lymphocytes to follicles. It was reported that the B cell chemokine CXCL13 is elevated in MS serum and cerebrospinal fluid. CXCL13 is also regarded as a proposed serum biomarker of synovitis in RA( Rheumatoid arthritis). CXCL13 consists of a 22-aa signal peptide and a 87-aa mature poly-peptide.

Publikationen

Species	Application	Title
human	IHC	Sci Adv; Defined lifestyle and germline factors predispose Asian populations to gastric cancer.; Authors - Akihiro Suzuki
human	IF	Nat Commun; B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.; Authors - Johannes Griss
human	IHC	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi
human	WB	Aging (Albany NY); Immune profiling reveals prognostic genes in high-grade serous ovarian cancer.; Authors - Yong Wu
human	IHC,IF	J Immunol; Villitis of unknown etiology is associated with a distinct pattern of chemokine up-regulation in the feto-maternal and placental compartments: implications for conjoint maternal allograft rejection and maternal anti-fetal graft-versus-host disease.; Authors - Kim Mi Jeong MJ
human	IHC，IF	Front Immunol; A Standardized Analysis of Tertiary Lymphoid Structures in Human Melanoma: Disease Progression- and Tumor Site-Associated Changes With Germinal Center Alteration.; Authors - Franziska Werner