CXCL14 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CXCL14 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10468-1-AP

Synonyme

C-X-C motif chemokine 14, Chemokine BRAK, C X C motif chemokine 14, BRAK, bolekine

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Galerie der Validierungsdaten

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  IHC staining of human liver using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 10468-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10468-1-AP (CXCL14 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10468-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CXCL14 antibody (10468-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolonkarzinomgewebe, humanes Magengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Produktinformation

10468-1-AP bindet in IHC, IF/ICC, ELISA CXCL14 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL14 fusion protein Ag0747
• Vollständiger Name: chemokine (C-X-C motif) ligand 14
• Berechnetes Molekulargewicht: 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC003513
• Gene symbol: CXCL14
• Gene ID (NCBI): 9547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL 14 (C-X-C motif chemokine 14) is a small inducible cytokine, and may be a potent chemoattractant for neutrophils. CXCL14 possesses a destruction box (D-box) domain which acts as a recognition signal for degradation via the ubiquitin-proteasome pathway. CXCL14 is widely expressed in normal tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas without inflammatory stimuli. It is dispensable for dendritic cell function and localization within peripheral tissues. In localized prostate cancer, CXCL14 mRNA was significantly upregulated and positively correlated with Gleason score.

Publikationen

Species	Application	Title
human	IHC	Transl Res; Novel role of CXCL14 in modulating STAR expression in luteinized granulosa cells: implication for progesterone synthesis in PCOS patients.; Authors - Jia Qi
	IF	Dis Model Mech; Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.; Authors - Kyoung-In Cho
human		Front Pharmacol; Aberrant ROS Mediate Cell Cycle and Motility in Colorectal Cancer Cells Through an Oncogenic CXCL14 Signaling Pathway.; Authors - Jun Zeng ; KD Validated
human	IHC	Int Immunopharmacol; Comprehensive analysis of the expression and prognostic value of CXC chemokines in colorectal cancer.; Authors - Li Yu
human	IHC	Thyroid; Iodide-induced chemokines and genes related to immunological function in cultured human thyroid follicles in the presence of thyrotropin.; Authors - Yamazaki Kazuko K
human	IHC	J Transl Med; Chemokine CXCL14 is associated with prognosis in patients with colorectal carcinoma after curative resection.; Authors - Zeng Jun J ; KD Validated