CXCL16 Polyklonaler Antikörper

CXCL16 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 29589-1-AP

Synonyme

C X C motif chemokine 16, CXCL16, CXCLG16, SCYB16, Small inducible cytokine B16, SR PSOX, SRPSOX, Transmembrane chemokine CXCL16

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  WB analysis using 29589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29589-1-AP (CXCL16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29589-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29589-1-AP (CXCL16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

29589-1-AP bindet in WB, ELISA CXCL16 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCL16 fusion protein Ag30981
• Vollständiger Name: chemokine (C-X-C motif) ligand 16
• Berechnetes Molekulargewicht: 273 aa, 30 kDa
• Beobachtetes Molekulargewicht: 28 kDa , 50-55 kDa
• GenBank-Zugangsnummer: BC017588
• Gene symbol: CXCL16
• Gene ID (NCBI): 58191
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCL16 was discovered as a ligand for CXC chemokine receptor (CXCR) 6. CXCL16 is a membrane-bound chemokine that consists of four distinct domains: the chemokine domain, mucin-like domain, transmembrane domain and ad cytoplasmic. After cleavage, soluble CXCL16 acts as a chemoattractant for activated CD8 T cells, NKT cells and Th1-polarized T cells that express CXCR6. CXCL16 expression has been studied mainly in macrophages and dendritic cells. CXCL16 production by non-immune cells remains controversial. CXCL16 was expressed as two different forms: a 50 kDa form and a 28 kDa form, and the 50 kDa may be the glycosylated form (PMID: 17855433, 33552057). (PMID: 17855433, 33552057).

Publikationen

Species	Application	Title
human	WB	Free Radic Biol Med; 8-Oxoguanine Modifications in Peripheral Leukocyte microRNAs Promote Age-related Inflammation by Remodeling Gene Regulatory Networks; Authors - Ming-Ming Han