CXCL2 Rekombinanter Antikörper

CXCL2 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242007H8

Kat-Nr. : 84645-4-PBS

Synonyme

C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2, Mip-2, Scyb2

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  Sandwich ELISA standard curve of MP01474-1

  Sandwich ELISA standard curve of MP01474-1, Mouse CXCL2 Recombinant Matched Antibody Pair - PBS only. 84645-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2239. 84645-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

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  Sample test of MP01474-1

  Serum of fifteen individual healthy human donors was measured. The CXCL2 concentration of detected samples was determined to be 134.5 pg/mL with a range of 57.0-304.7 pg/mL.

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  Sample test of MP01474-1

  J774A.1 cells were cultured unstimulated or stimulated with 20 ng/mL IFN-γ and 1 μg/mL LPS for 1 day. The mean CXCL2 concentration was determined to be 71.93 ng/mL in unstimulated supernatant, 2,704.98 ng/mL in stimulated supernatant.

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  Sample test of MP01474-1

  Mouse lung were rinsed with enough PBS to cover the organs and kept on ice. Tissue was homogenized with a tissue homogenizer and cultured in RPMI 1640 supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin sulfate for 3 days. Aliquots of the cell culture supernates were removed and assayed for mouse CXCL2, and measured 20.41 ng/mL.

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  Cytometric bead array standard curve of MP01474-1

  Cytometric bead array standard curve of MP01474-1, MOUSE CXCL2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84645-3-PBS. Detection antibody: 84645-4-PBS. Standard: Eg2239. Range: 0.625-40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

84645-4-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CXCL2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL2 fusion protein Eg2239
• Vollständiger Name: chemokine (C-X-C motif) ligand 2
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_009140
• Gene symbol: Cxcl2
• Gene ID (NCBI): 20310
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.