CXCL2 Rekombinanter Antikörper

CXCL2 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

242007G5

Kat-Nr. : 98259-1-PBS

Synonyme

242007G5, C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2, Mip-2

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  FC experiment of RAW 264.7 using 98259-1-RR (same clone as 98259-1-PBS)

  1x10^6 untreated or LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse CXCL2 Rabbit RecAb (98259-1-RR, Clone: 242007G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98259-1-PBS in a different storage buffer formulation.

• 

  FC experiment of RAW 264.7 using 98259-1-RR (same clone as 98259-1-PBS)

  1x10^6 LPS and Brefeldin A treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse CXCL2 Rabbit RecAb (98259-1-RR, Clone: 242007G5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse CXCL2 Rabbit Recombinant Antibody (98259-1-RR, Clone: 242007G5) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98259-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98259-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98259-1-RR against Mouse CXCL2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98259-1-PBS bindet in FC (Intra) CXCL2 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL2 fusion protein Eg2239
• Vollständiger Name: chemokine (C-X-C motif) ligand 2
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_009140
• Gene symbol: Cxcl2
• Gene ID (NCBI): 20310
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).