CXCL4/PF4 Rekombinanter Antikörper

CXCL4/PF4 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

243087F2

Kat-Nr. : 85647-1-RR

Synonyme

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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  WB analysis of mouse serum using 85647-1-RR

  mouse serum was subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 85647-1-RR

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 85647-1-RR (CXCL4/PF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85647-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84647-1-RR against Human CXCL4/PF4 were performed. The affinity constant is 0.625 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mouse serum, human peripheral blood platelets

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85647-1-RR bindet in WB, ELISA CXCL4/PF4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL4/PF4 fusion protein Eg2129
• Vollständiger Name: platelet factor 4
• Berechnetes Molekulargewicht: 11 kDa
• Beobachtetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_002619.4
• Gene symbol: PF4
• Gene ID (NCBI): 5196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation