CXCL4/PF4 Rekombinanter Antikörper

CXCL4/PF4 Rekombinant Antikörper für FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243087D5

Kat-Nr. : 98410-2-PBS

Synonyme

CXCL4, PF4, SCYB4, C X C motif chemokine 4, C-X-C motif chemokine 4

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  Sandwich ELISA sample test of MP02040-2

  Serum of sixteen individual healthy human donors was measured. The CXCL4/PF4 concentration of detected samples was determined to be 14,975.2 ng/mL with a range of 7,843.8-26,666.8 pg/mL.

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  Sandwich ELISA standard curve of MP02040-2

  Sandwich ELISA standard curve of MP02040-2, Human CXCL4/PF4 Recombinant Matched Antibody Pair - PBS only. 85647-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2129. 98410-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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  FC experiment of human PBMCs using 98410-2-RR (same clone as 98410-2-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-2-RR, Clone: 243087D5) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98410-2-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98410-2-RR (same clone as 98410-2-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL4/PF4 Rabbit RecAb (98410-2-RR, Clone: 243087D5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated. This data was developed using the same antibody clone with 98410-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02040-2

  Cytometric bead array standard curve of MP02040-2, CXCL4/PF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85647-3-PBS. Detection antibody: 98410-2-PBS. Standard: Eg2129. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

98410-2-PBS bindet in FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA CXCL4/PF4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CXCL4/PF4 fusion protein Eg2129
• Vollständiger Name: platelet factor 4
• Berechnetes Molekulargewicht: 11 kDa
• GenBank-Zugangsnummer: NM_002619.4
• Gene symbol: PF4
• Gene ID (NCBI): 5196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CXCL4, also known as platelet factor 4 (PF-4), is the oldest member of the chemokine family. CXCL4 is a chemokine produced by activated platelets and immune cells involved in pathological conditions such as cancer, infections and inflammatory diseases like systemic sclerosis (SSc), rheumatoid arthritis (RA) and psoriatic arthritis (PsA), among others. CXCL4 is present in the α-granules of platelets in amounts that constitute up to 2% platelet protein mass. CXCL4 plays a determinant role in distinct physiological processes such as in hematopoiesis, angiogenesis, coagulation and modulation of immune responses. For instance, CXCL4 can prevent monocyte apoptosis and promote cell survival, induces the production of TNF and reactive oxygen species (ROS) and promotes monocyte differentiation into a unique macrophage-like phenotype.