CXCL8/IL-8 Rekombinanter Antikörper

CXCL8/IL-8 Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240889B2

Kat-Nr. : 98137-1-PBS

Synonyme

CXCL8, IL8, IL-8, C-X-C motif chemokine 8, C-X-C motif

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  FC experiment of human PBMCs using 98137-1-RR (same clone as 98137-1-PBS)

  1x10^6 untreated or IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14 Rabbit Recombinant Antibody (CL647-98040, Clone: 230332D7). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98137-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98137-1-RR (same clone as 98137-1-PBS)

  1x10^6 IFN gamma, LPS and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human CXCL8/IL-8 Rabbit Recombinant Antibody (98137-1-RR, Clone: 240889B2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98137-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98137-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98137-1-RR against Human CXCL8/IL-8 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

98137-1-PBS bindet in FC (Intra) CXCL8/IL-8 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: interleukin 8
• Berechnetes Molekulargewicht: 99 aa, 11 kDa
• GenBank-Zugangsnummer: BC013615
• Gene symbol: IL-8
• Gene ID (NCBI): 3576
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Interleukin 8 (IL-8), also known as CXCL8, is a CXC chemokine family member. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.