CXCR3 Rekombinanter Antikörper

CXCR3 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241790E9

Kat-Nr. : 84655-5-PBS

Synonyme

CMKAR3, CKR-L2, CKR L2, CD183, CD182

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  WB analysis using 84655-5-RR (same clone as 84655-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84655-5-RR (CXCR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84655-5-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 84655-5-RR (same clone as 84655-5-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 84655-5-RR (CXCR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84655-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84655-5-RR (same clone as 84655-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84655-5-RR (CXCR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84655-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84655-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84655-5-RR against Human CXCR3 were performed. The affinity constant is 1.84 nM.

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Geprüfte Anwendungen

Produktinformation

84655-5-PBS bindet in WB, IHC, Indirect ELISA CXCR3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: chemokine (C-X-C motif) receptor 3
• Berechnetes Molekulargewicht: 368 aa, 41 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC034403
• Gene symbol: CXCR3
• Gene ID (NCBI): 2833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of several cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. The binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively, spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as a functional receptor for CXCL4 (PMID:12782716).