CXCR3B-specific Monoklonaler Antikörper

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• KD/KO Validated

CXCR3B-specific Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B2D6

Kat-Nr. : 60065-1-PBS

Synonyme

CXCR3, CMKAR3, CKR-L2, CKR L2, CD183

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  WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)

  MOLT-4 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60065-1-Ig (same clone as 60065-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 60065-1-Ig (same clone as 60065-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 60065-1-Ig (same clone as 60065-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 60065-1-Ig (same clone as 60065-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CXCR3B-specific antibody (60065-1-Ig, Clone: 1B2D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60065-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA CXCR3B-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: chemokine (C-X-C motif) receptor 3
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: AF469635
• Gene symbol: CXCR3
• Gene ID (NCBI): 2833
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.