CXCR4 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CXCR4 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(62)

CloneNo.

4B5E4

Rezensionen (1)

Kat-Nr. : 60042-1-Ig

Synonyme

CD184, C-X-C chemokine receptor type 4, CXC R4, CXCR 4, CXC-R4

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  WB analysis using 60042-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 60042-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 60042-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 60042-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60042-1-Ig (CXCR4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60042-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60042-1-Ig (CXCR4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Daudi-Zellen, Jurkat-Zellen, K-562-Zellen, Raji-Zellen, Ramos-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 45 publications below
• IHC: See 12 publications below
• IF: See 16 publications below
• CoIP: See 2 publications below

Produktinformation

60042-1-Ig bindet in WB, IHC, IF, CoIP, ELISA CXCR4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CXCR4 fusion protein Ag1528
• Vollständiger Name: chemokine (C-X-C motif) receptor 4
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• Beobachtetes Molekulargewicht: 60~70 kDa
• GenBank-Zugangsnummer: BC020968
• Gene symbol: CXCR4
• Gene ID (NCBI): 7852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

Publikationen

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
human	WB	Adv Mater; Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment; Authors - Yuewen Zhai
mouse	WB	Adv Sci (Weinh); Mesenchymal Stem Cell Membrane-Camouflaged Liposomes for Biomimetic Delivery of Cyclosporine A for Hepatic Ischemia-Reperfusion Injury Prevention; Authors - Haitian Chen
mouse	WB	Nat Commun; HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity.; Authors - Hiroaki Semba
mouse	WB,IF	J Exp Med; CXCR4 regulates Plasmodium development in mouse and human hepatocytes.; Authors - Hironori Bando ; KD Validated
human	WB	Cancer Lett; YTHDF1 promotes the osteolytic bone metastasis of breast cancer via inducing EZH2 and CDH11 translation; Authors - Shuoer Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rui (Verified Customer) (05-08-2025)

It was not working

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500