CXCR4 Rekombinanter Antikörper

CXCR4 Rekombinant Antikörper für IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241734B2

Kat-Nr. : 84904-1-RR

Synonyme

241734B2, CD184, C-X-C chemokine receptor type 4, CXC R4, CXCR 4

---

• 

  IF Staining of mouse testis using 84904-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 84904-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of Jurkat using 84904-1-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:125-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84904-1-RR bindet in IF/ICC, IF-P, ELISA CXCR4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: chemokine (C-X-C motif) receptor 4
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• GenBank-Zugangsnummer: BC020968
• Gene symbol: CXCR4
• Gene ID (NCBI): 7852
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).