CXCR4/CD184 Rekombinanter Antikörper

CXCR4/CD184 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

241735F8

Kat-Nr. : APC-98175

Synonyme

CXCR4, 241735F8, CD184, C-X-C chemokine receptor type 4, CXC R4

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  FC experiment of human PBMCs using APC-98175

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (FITC-65562, Clone: 4G7), and 5 ul APC Anti-Human CXCR4/CD184 Rabbit RecAb (APC-98175, Clone: 241735F8) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human PBMCs using APC-98175

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CXCR4/CD184 Rabbit RecAb (APC-98175, Clone: 241735F8) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-98175 bindet in FC CXCR4/CD184 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: chemokine (C-X-C motif) receptor 4
• Berechnetes Molekulargewicht: 352 aa, 40 kDa
• GenBank-Zugangsnummer: BC020968
• Gene symbol: CXCR4
• Gene ID (NCBI): 7852
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).