CXCR5 Polyklonaler Antikörper

CXCR5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28239-1-AP

Synonyme

BLR1, Burkitt lymphoma receptor 1, CD185, CXC R5, CXCR 5, CXCR5, MDR 15, MDR15, Monocyte derived receptor 15

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Galerie der Validierungsdaten

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  WB analysis using 28239-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28239-1-AP (CXCR5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 28239-1-AP

  WB result of CXCR5 antibody (28239-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXCR5 transfected Jurkat cells.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Mausmilzgewebe, Rattenmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

28239-1-AP bindet in WB, ELISA CXCR5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CXCR5 fusion protein Ag28271
• Vollständiger Name: chemokine (C-X-C motif) receptor 5
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: NM_001716
• Gene symbol: CXCR5
• Gene ID (NCBI): 643
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).